Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS Triton X100 (0.5%;10min)
Other product details
Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
This antibody appears to recognize p53 phosSer15 following DNA induced damage (+ Bleomycin). However, in untreated HeLa cells it also appears to recognize an epitope associated with the centrosomes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Jul 02 2007