Application
Western blot
Sample
Human Cell lysate - nuclear (kidney)
Loading amount
20 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing (4-12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Cy5 Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Dilution
1/5000
Detection
Detection method
Fluorescence
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 40, 80 kDa
Additional data
Additional Notes
Bands were really weak!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 13 2012