概述

  • 产品名称
    Anti-p53 DINP1抗体
    参阅全部 p53 DINP1 一抗
  • 描述
    兔多克隆抗体to p53 DINP1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (

    MFQRLNKMFVGEVS

    ) corresponding to amino acids 1 to 14 of human p53DINP1. This sequence is identical between alpha and beta, and differs by one amino acid from those of mouse. (Peptide available as ab9776.)

  • 阳性对照
    • Human lung tissue lysate. Also human spleen, mouse liver and kidney tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab9775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Can be blocked with Human p53 DINP1 peptide (ab9776). Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, and mouse liver and kidney tissue lysates.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis.
  • 组织特异性
    Ubiquitously expressed.
  • 细胞定位
    Nucleus > PML body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434M1317 antibody
    • FLJ22139 antibody
    • p53 damage inducible nuclear protein 1 antibody
    • p53 dependent damage inducible nuclear protein 1 antibody
    • p53 inducible nuclear protein 1 antibody
    • p53 inducible p53DINP1 antibody
    • p53-dependent damage-inducible nuclear protein 1 antibody
    • p53DINP1 antibody
    • SIP antibody
    • Stress induced protein antibody
    • Stress-induced protein antibody
    • T53I1_HUMAN antibody
    • Teap antibody
    • TP53 DINP1 antibody
    • TP53 INP1 antibody
    • TP53DINP1 antibody
    • TP53INP1 antibody
    • TP53INP1A antibody
    • TP53INP1B antibody
    • Tumor protein p53 inducible nuclear protein 1 antibody
    • Tumor protein p53-inducible nuclear protein 1 antibody
    see all

图片

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling p53 DINP1 with Anti-p53 DINP1 antibody (ab9775) at 5μg/ml.
  • Western blot analysis of p53DINP1 expression in human lung tissue lysate with anti-p53DINP1 (ab9775) at 2 µg/ml in the absence (lane 1) or presence (lane 2) of blocking peptide (ab9776).

    Western blot analysis of p53DINP1 expression in human lung tissue lysate with anti-p53DINP1 (ab9775) at 2 µg/ml in the absence (lane 1) or presence (lane 2) of blocking peptide (ab9776).
  • Immunohistochemical staining (paraffin embedded) of mouse liver using anti-p53 DINP1 at 2 µg/ml. Immunohistochemical staining (paraffin embedded) of mouse liver using anti-p53 DINP1 at 2 µg/ml.

文献

This product has been referenced in:
  • Zhang J  et al. microRNA-155 acts as an oncogene by targeting the tumor protein 53-induced nuclear protein 1 in esophageal squamous cell carcinoma. Int J Clin Exp Pathol 7:602-10 (2014). WB, IHC-P ; Human . Read more (PubMed: 24551280) »
  • Polioudakis D  et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. Nucleic Acids Res 41:2239-54 (2013). WB . Read more (PubMed: 23303785) »

See all 4 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (Primary macrophage)
Gel Running Conditions
Reduced Denaturing (Bis-tris gradient gel, MOPS buffer)
Loading amount
85 µg
Specification
Primary macrophage
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. David Gregory

Verified customer

提交于 May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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