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Recombinant full length protein corresponding to Human p53. Recombinant human wild type p53 protein expressed in E. coli.
Database link: P04637
Alternative versions available:
Our Abpromise guarantee covers the use of ab80645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.
Use at an assay dependent concentration.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa. Ab 1-2µg/ml for 2hrs at RT.|
ab80645 at 1/500 staining lesioned skin sections by IHC-P. The tissue was fixed in formaldehyde and citric acid was used as antigen retrieval solution. The tissue sections were blocked using 1% BSA followed by incubation with ab80645 for 2 hours. A HRP conjugated goat anti-mouse monoclonal antibody at 1/200 was used as the secondary. Skin lesion shows clean, clear strong positivity in a subset of nuclei.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"