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Recombinant full length protein corresponding to Human p53. Recognizes defined epitope (aa 16-25) on human p53.
Database link: P04637
Our Abpromise guarantee covers the use of ab7757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200. 1/200 - please refer to abreview for further protocol details.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
Image courtesy of an Abreview submitted by Santhosh Gopal.
p53 was immunoprecipitated using 0.5mg T47D whole cell extract, 5µg of Rabbit polyclonal to Mouse monoclonal to p53 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, T47D whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7757.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 53kDa; p53