Application
Flow Cytometry
Fixation
Methanol
Permeabilization
Yes - 0.1% Tween
Sample
Human Cell (MDA-MB-231)
Specification
MDA-MB-231
Gating Strategy
Live cells gated
Preparation
Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Sample buffer: Enzyme free
Other product details
Concentration
1 µg/cells
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS + 0.2% BSA + 0.1% Sodium azide
Secondary antibody
Name
Non-Abcam antibody was used: Chicken anti-mouse IgG PE
Host species: Chicken
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Chicken
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
Additional Notes
The purple plot represents the isotype control and the green plot represents p53 in MDA-MB-231 cells.
Abcam response
Thank you for sharing this data with us. ab26 has previously been tested in flow cytometry on HeLa cells (please see image on datasheet). However, based on this abreview, it is clear that this antibody does not work in flow on MDA-MB-231 cells, under these experimental conditions. Abcam would like to hear from other customers using this antibody for flow cytometry.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 29 2014