Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Human Cell lysate - whole cell (HCT116)
Specification
HCT116
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.5% Tween + Azide
Secondary antibody
Name
Non-Abcam antibody was used: Odyssey
Host species: Goat
Clonality: Polyclonal
Conjugation: Chromeo™ 505
Host species: Goat
Clonality: Polyclonal
Conjugation: Chromeo™ 505
Dilution
1/10000
Detection
Detection method
Odyssey machine
Bands
Specific: 53 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 30 2013