Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (13%)
Sample
Human Cell lysate - whole cell (HCT116 human colon carcinoma cell line)
Specification
HCT116 human colon carcinoma cell line
Treatment
DMSO (Ctl) and 10 ´M irinotecan (CPT-11) treatment for 24 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2 % milk in TBS-T
Dilution
1/1000
Secondary antibody
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor 680)
Dilution
1/10000
Detection
Detection method
LiCor Odyssey
Bands
Specific: 55 kDa Non-specific: 90 kDa
Exposure
3 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christian Marx
Verified customer
提交于 Nov 03 2014