Anti-P4HA2抗体(ab70887)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to P4HA2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 250 and 300 of human P4HA2. (NP_004190.1)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • 序列相似性
    Belongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • 细胞定位
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
    • 4 PH alpha 2 antibody
    • 4-PH alpha-2 antibody
    • C-P4Halpha(II) antibody
    • collagen prolyl 4-hydroxylase alpha(II) antibody
    • P4HA2 antibody
    • P4HA2_HUMAN antibody
    • procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II antibody
    • procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2 antibody
    • Procollagen-proline antibody
    • procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
    • Prolyl 4 hydroxylase alpha 2 subunit antibody
    • Prolyl 4 hydroxylase subunit alpha 2 [Precursor] antibody
    • Prolyl 4-hydroxylase subunit alpha-2 antibody
    • Prolyl 4-hydroxylase, alpha polypeptide II antibody
    see all

图片

  • All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa
    Additional bands at: 28 kDa, 33 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from 9L cells
    Lane 2 : Whole cell lysate prepared from PC12 cells
    Lane 3 : Whole cell lysate prepared from RIE-1 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa
    Additional bands at: 35 kDa (possible degradation product)


    Exposure time: 5 minutes


    Good immunoreactivity observed in RIE-1 cell lysate that is consistent with the mobility of full-length P4HA2. A faster migrating species of ~35 kDa has been commonly observed with other antibodies (including this one) that could be a degradation product or alternatively spliced form of P4HA2. Further charcaterisation is required.

    See Abreview

文献

ab70887 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat cell lines)
Loading amount
20 µg
Specification
Rat cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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提交于 Feb 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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