Anti-p400抗体- ChIP Grade (ab70301)

概述

  • 产品名称Anti-p400抗体- ChIP Grade
    参阅全部 p400 一抗
  • 描述
    兔多克隆抗体to p400 - ChIP Grade
  • 经测试应用适用于: WB, IP, ChIP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 3075 and 3124 of human p400

  • 阳性对照
    • HeLa whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Detects a band of approximately 470 kDa (predicted molecular weight: 344 kDa).
IP Use at 2 µg/mg of lysate.
ChIP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HSA domain.
    Contains 1 Myb-like domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAG repeat protein 32 antibody
    • CAGH 32 antibody
    • CAGH32 antibody
    • DKFZP434I225 antibody
    • Domino homolog antibody
    • E1A binding protein p400 antibody
    • E1A-binding protein p400 antibody
    • EP 400 antibody
    • EP400 antibody
    • EP400_HUMAN antibody
    • FLJ42018 antibody
    • FLJ45115 antibody
    • hDomino antibody
    • KIAA1498 antibody
    • KIAA1818 antibody
    • p400 kDa SWI2/SNF2 related protein antibody
    • p400 kDa SWI2/SNF2-related protein antibody
    • P400 SWI2/SNF2 related protein antibody
    • TNRC 12 antibody
    • TNRC12 antibody
    • Trinucleotide repeat containing 12 antibody
    • Trinucleotide repeat containing gene 12 protein antibody
    • Trinucleotide repeat-containing gene 12 protein antibody
    see all

Anti-p400 antibody - ChIP Grade 图像

  • All lanes : Anti-p400 antibody - ChIP Grade (ab70301) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 344 kDa
    Observed band size : 470 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Human p400 by Western Blot of Immunprecipitate. Lane 1: Irrelevant antibody used for precipitation. Lane 2: ab70301 at 0.04µg/ml staining p400 in HeLa whole cell lysates immunoprecipitated using ab70301 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: Control IgG used for precipitation. Detection: Chemiluminescence with exposure time 10 seconds.
  • ab70301 (1/200) staining p400 in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized using 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • ICC/IF image of ab70301 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70301, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-p400 antibody - ChIP Grade (ab70301)参考文献

This product has been referenced in:
  • Pérez-García V  et al. Cell activation-induced phosphoinositide 3-kinase alpha/beta dimerization regulates PTEN activity. Mol Cell Biol 34:3359-73 (2014). WB ; Mouse . Read more (PubMed: 24958106) »
  • Bellucci L  et al. Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z. PLoS One 8:e54102 (2013). ChIP ; Human . Read more (PubMed: 23349794) »

See all 5 Publications for this product

Product Wall

Due to the high molecular weight of this protein, , we recommend using NuPAGE 3-8% tris-Acetate Gels from Novex (formerly Invitrogen) Catalog # EA03752Box, Novex Tris Acetate SDS Running Buffer, and Novex Transfer Buffer-20X. Run the transfer apparatus...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Sep 03 2012

Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. How...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Colon cancer cell line)
Specification Colon cancer cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Detection step Real-time PCR
Negative control 1.ChIP with IgG.
2.ChIP at intron region
Username

Dr. santosh chauhan

Verified customer

提交于 Aug 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"