概述

  • 产品名称Anti-p400抗体
    参阅全部 p400 一抗
  • 描述
    兔多克隆抗体to p400
  • 特异性ab5201 specifically reacts with p400. p400 is immunoprecipiated with Tip60-TAP and there is no reactivity with Mock or unrelated TAP-tagged proteins (see the Western blot image).
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from residues 200 - 300 of Human p400.

    .

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Tip60-TAP Transfected HeLa.

      This antibody gave a positive signal in the following cell lines: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab5201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 19000314
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Predicted molecular weight: 344 kDa.

靶标

  • 功能Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HSA domain.
    Contains 1 Myb-like domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAG repeat protein 32 antibody
    • CAGH 32 antibody
    • CAGH32 antibody
    • DKFZP434I225 antibody
    • Domino homolog antibody
    • E1A binding protein p400 antibody
    • E1A-binding protein p400 antibody
    • EP 400 antibody
    • EP400 antibody
    • EP400_HUMAN antibody
    • FLJ42018 antibody
    • FLJ45115 antibody
    • hDomino antibody
    • KIAA1498 antibody
    • KIAA1818 antibody
    • p400 kDa SWI2/SNF2 related protein antibody
    • p400 kDa SWI2/SNF2-related protein antibody
    • P400 SWI2/SNF2 related protein antibody
    • TNRC 12 antibody
    • TNRC12 antibody
    • Trinucleotide repeat containing 12 antibody
    • Trinucleotide repeat containing gene 12 protein antibody
    • Trinucleotide repeat-containing gene 12 protein antibody
    see all

Anti-p400 antibody 图像

  • ICC/IF image of ab5201 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 10 min) and incubated with the antibody (ab5201, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • All lanes : Anti-p400 antibody (ab5201)

    Lane 1 : Mock Transfected HeLa
    Lane 2 : Tip60-TAP Transfected HeLa
    Lane 3 : ING4-TAP Transfected HeLa


    Predicted band size : 344 kDa

    Rabbit polyclonal to p400 used at 1/1000. p400 is part of the Tip60/NuA4 HAT complex.

    Nuclear extracts from Mock (1), Tip60 (2) and an unrelated TAP-tagged protein (3) were purified by tandem affinity purification. No non-specific bands are observed.

    Whilst it is our normal policy to display the full gel for Western Blots, in this case we received a strip image from an academic collaborator. However, we still feel this image provides useful information on ab5201.

    Image from PMID:16387653 Western blot analysis of p400 antibody using TAP purifications of ING4 and Tip60 that shows two distinct complexes

Anti-p400 antibody (ab5201)参考文献

This product has been referenced in:
  • Taty-Taty GC  et al. Control of alternative end joining by the chromatin remodeler p400 ATPase. Nucleic Acids Res 44:1657-68 (2016). WB . Read more (PubMed: 26578561) »
  • Goldman JA  et al. Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z. J Biol Chem 285:4645-51 (2010). WB ; Human . Read more (PubMed: 19940112) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us. The immunogen can be purchased from Abcam, it is catalog ab13753.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 May 04 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Dec 20 2010

Thank you for your enquiry. It is difficult to predict the cross reactivity of this antibody given an 87% identity. The reactivity will largely depend on the sites of identity and the epitopes recognized by the polyclonal antisera. On this occa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"