Anti-p38 (phospho T180 + Y182)抗体(ab4822)

概述

  • 产品名称
    Anti-p38 (phospho T180 + Y182)抗体
    参阅全部 p38 一抗
  • 描述
    兔多克隆抗体to p38 (phospho T180 + Y182)
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Dog, Carp, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human p38 (phospho T180 + Y182). p38 is dually phosphorylated and therefore fully activated by MEK3 and MEK6 on threonine 180 and tyrosine 182 within the activation loop.
    Database link: Q16539
    (Peptide available as ab5253)

  • 阳性对照
    • HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% PBS, 50% Glycerol, 0.1% BSA

    BSA is IgG and protease free. PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/100.
ICC/IF 1/250.
WB 1/1000. Predicted molecular weight: 38 kDa.

靶标

  • 功能
    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • 组织特异性
    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

Anti-p38 (phospho T180 + Y182) antibody 图像

  • All lanes : Anti-p38 (phospho T180 + Y182) antibody (ab4822) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) exposed for 40 min with UV, cell lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 4 : A431 (human epidermoid carcinoma cell line) exposed for 40 min with UV, cell lysate
    Lane 5 : COLO 205 (human colon adenocarcinoma cell line) cell lysate
    Lane 6 : COLO 205 (human colon adenocarcinoma cell line) exposed for 40 min with UV, cell lysate
    Lane 7 : A549 (human lung carcinoma cell line) cell lysate
    Lane 8 : A549 (human lung carcinoma cell line) exposed for 40 min with UV, cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-Rabbit IgG HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 38 kDa
  • Paraffin-embedded human brain tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/100 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody. 

  • Paraffin-embedded human heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.

  • Paraffin-embedded rat heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.

  •  


  • Predicted band size : 38 kDa
    Peptide Competition: Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide

Anti-p38 (phospho T180 + Y182) antibody (ab4822)参考文献

This product has been referenced in:
  • Kuanpradit C  et al. Attenuation of UV-B exposure-induced inflammation by abalone hypobranchial gland and gill extracts. Int J Mol Med 39:1083-1090 (2017). WB ; Human . Read more (PubMed: 28358420) »
  • Kang K  et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep 6:22632 (2016). WB . Read more (PubMed: 26961159) »

See all 13 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
100 pM IL-1β
Specification
MDCK
Blocking step
BlockAce as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Username

令 中野

Verified customer

提交于 Jul 14 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (retina)
Specification
retina
Permeabilization
Yes - triton x100
Fixative
Paraformaldehyde
Username

Dr. Minzhong Yu

Verified customer

提交于 Feb 27 2015

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

S...

Read More

Thank you for contacting us. I am sorry to hear the customer has been having problems with this antibody. I think the right band is being detected at ~55 kDa. I would like to suggest some tips that might help improve the results: - Decr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Caco-2 epithelial colorectal adenocarcinoma)
Loading amount
10 µg
Specification
Human Caco-2 epithelial colorectal adenocarcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
20 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 25 2008

Thank you for your enquiry and patience. The concentration for this batch is 0.125 mg/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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