使用敲除细胞株进行验证RabMAb

Anti-p38抗体[E229] - BSA and Azide free (ab225534)

概述

  • 产品名称
    Anti-p38抗体[E229] - BSA and Azide free
    参阅全部 p38 一抗
  • 描述
    兔单克隆抗体[E229] to p38 - BSA and Azide free
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, Flow Cyt, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p38 aa 150-250 (internal sequence).
    Database link: Q16539

  • 阳性对照
    • WB: Jurkat, C6, NIH/3T3 or HeLa cell lysate. ICC/IF: NIH/3T3 cell lysate.
  • 常规说明

    ab225534 is PBS-buffered version of ab170099, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab170099 for protocols, dilutions, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab225534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.

靶标

  • 功能
    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • 组织特异性
    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

图片

  • This WB data was generated using the same anti-p38 antibody clone [E229] in a different buffer formulation (cat# ab170099).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: p38 knockout HAP1 cell lysate (20 µg) 
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg) 
    Lanes 1 - 4: Merged signal (red and green). Green - ab170099 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab170099 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab170099 and

    ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

文献

This product has been referenced in:
  • Ye S  et al. Thrombosis recanalization by paeoniflorin through the upregulation of urokinase-type plasminogen activator via the MAPK signaling pathway. Mol Med Rep 13:4593-8 (2016). WB ; Human . Read more (PubMed: 27082639) »
  • Li C  et al. LFG-500, a newly synthesized flavonoid, attenuates lipopolysaccharide-induced acute lung injury and inflammation in mice. Biochem Pharmacol 113:57-69 (2016). Read more (PubMed: 27206337) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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