概述

  • 产品名称
  • 描述
    兔多克隆抗体to p35
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Guinea pig, Cow, Cat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 255-304 (EAFWDRCLSV INLMSSKMLQ INADPHYFTQ VFSDLKNESG QEDKKRLLLG) of Mouse p35 (NP_034001.1).

  • 阳性对照
    • Mouse pancreas lysate.

性能

应用

Our Abpromise guarantee covers the use of ab123048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 组织特异性
    Brain and neuron specific.
  • 序列相似性
    Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻译后修饰
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 细胞定位
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD5R1_HUMAN antibody
    • CDK5 activator 1 antibody
    • CDK5P35 antibody
    • CDK5R antibody
    • CDK5R1 antibody
    • Cyclin dependent kinase 5 regulatory subunit 1 antibody
    • Cyclin-dependent kinase 5 activator 1 antibody
    • Cyclin-dependent kinase 5 regulatory subunit 1 antibody
    • NCK 5A antibody
    • Neuronal CDK5 activator antibody
    • p23 antibody
    • p25 antibody
    • p35 antibody
    • Regulatory partner for CDK5 kinase antibody
    • Tau protein kinase II 23 kDa subunit antibody
    • TPKII regulatory subunit antibody
    see all

图片

  • Anti-p35 antibody (ab123048) at 1 µg/ml + Mouse pancreas lysate at 10 µg

    Predicted band size : 34 kDa

实验方案

文献

ab123048 has not yet been referenced specifically in any publications.

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