概述

  • 产品名称Anti-P2X3抗体
    参阅全部 P2X3 一抗
  • 描述
    兔多克隆抗体to P2X3
  • 经测试应用适用于: WB, IHC-Fr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Non Human Primates
  • 免疫原

    Synthetic peptide:

    VEKQSTDSGAYSIGH

    , corresponding to amino acids 383-397 of C-terminus of Rat P2X3.

性能

应用

Our Abpromise guarantee covers the use of ab10269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 47.6 kDa.
IHC-Fr 1/1000.
ICC 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-P2X3 antibody 图像

  • Image A: ab10248, at a dilution of 1/1000, staining P2X3 (green) in rat dorsal root ganglia sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Other structures visualized with anti-ASIC3 (red). Image B: Structures in rat dorsal root ganglia sections visualized with anti-ASIC3 (red) and anti-TRPV1 (green). Double-labeled cells are yellow or orange.
  • ICC/IF image of ab10269 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10269, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-P2X3 antibody (ab10269)参考文献

This product has been referenced in:
  • Usoskin D  et al. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci 18:145-53 (2015). Read more (PubMed: 25420068) »

See 1 Publication for this product

Product Wall

Thank you for contacting us regarding anti-P2X3 antibody (ab10269).


Unfortunately , as discussed over the phone, the exact concentration of ab10269 has not been determined. This is quite common with antibody preparations which are suppl...

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Thank you for contacting us.

The antibody is a whole anti serum, specifc IgG is not purified so we do not know the conc. of this antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Spinal cord lysate)
Loading amount 40 µg
Specification Spinal cord lysate
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

提交于 Jun 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"