概述

  • 产品名称Anti-P2X2抗体
    参阅全部 P2X2 一抗
  • 描述
    兔多克隆抗体to P2X2
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cat
  • 免疫原

    Synthetic peptide, corresponding to amino acids 55-104 of Human P2X2

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab28469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 45-55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/2500.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.
  • 疾病相关Deafness, autosomal dominant, 41
  • 序列相似性Belongs to the P2X receptor family.
  • 细胞定位Cell membrane. Localizes to the apical membranes of hair cells in the organ of Corti.
  • Information by UniProt
  • 数据库链接
  • 形式There are 7 isoforms produced by alternative splicing.
  • 别名
    • ATP receptor antibody
    • MGC129601 antibody
    • P2RX 2 antibody
    • P2RX2 antibody
    • P2RX2_HUMAN antibody
    • P2X purinoceptor 2 antibody
    • P2X Receptor subunit 2 antibody
    • P2X2 antibody
    • P2X2a antibody
    • Purinergic receptor antibody
    • Purinergic receptor P2X ligand gated ion channel 2 antibody
    • Purinergic receptor P2X2 isoform A antibody
    • Purinergic receptor P2X2 isoform B antibody
    • Purinergic receptor P2X2 isoform C antibody
    • Purinergic receptor P2X2 isoform D antibody
    • Purinergic receptor P2X2 isoform H antibody
    • Purinergic receptor P2X2 isoform I antibody
    see all

Anti-P2X2 antibody 图像



  • Predicted band size : 45-55 kDa
    Observed band size : 49,55 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab28469 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28469, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-P2X2 antibody (ab28469)参考文献

ab28469 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Urinary bladder)
Loading amount 10 µg
Specification Urinary bladder
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Oct 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"