概述

  • 产品名称Anti-P2X1抗体
    参阅全部 P2X1 一抗
  • 描述
    兔多克隆抗体to P2X1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human P2X1.

    (Peptide available as ab91609.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Brain, Heart, Testis

性能

应用

Our Abpromise guarantee covers the use of ab74058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明IHC-P: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Seems to be linked to apoptosis, by increasing the intracellular concentration of calcium in the presence of ATP, leading to programmed cell death.
    • 序列相似性Belongs to the P2X receptor family.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP receptor antibody
      • H.sapiens mRNA for ATP receptor antibody
      • P2 RX1 antibody
      • P2rx1 antibody
      • P2RX1 protein antibody
      • P2RX1_HUMAN antibody
      • P2X purinoceptor 1 antibody
      • P2X receptor subunit 1 antibody
      • P2X1 antibody
      • P2X1 receptor antibody
      • Purinergic receptor antibody
      • Purinergic receptor P2X ligand gated ion channel 1 antibody
      • Purinergic receptor P2X1 antibody
      see all

    Anti-P2X1 antibody 图像

    • All lanes : Anti-P2X1 antibody (ab74058) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Human heart tissue lysate - total protein (ab29431)
      Lane 3 : Human testis tissue lysate - total protein (ab30257)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 45 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes
    • IHC image of ab74058 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74058, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-P2X1 antibody (ab74058)参考文献

    ab74058 has not yet been referenced specifically in any publications.

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