概述

  • 产品名称Anti-P2RX7抗体
    参阅全部 P2RX7 一抗
  • 描述
    山羊多克隆抗体to P2RX7
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Dog
  • 免疫原

    Synthetic peptide:

    YETNKVTRIQSMNY

    by a Cysteine residue linker, corresponding to N terminal amino acids 13-26 of Human P2RX7 (NP_002553.2)

  • 阳性对照
    • Human brain frontal cortex lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储���液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37, 70 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

靶标

  • 功能Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
  • 组织特异性Widely expressed with highest levels in brain and immune tissues.
  • 序列相似性Belongs to the P2X receptor family.
  • 翻译后修饰Phosphorylation results in its inactivation.
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP receptor antibody
    • P2rx7 antibody
    • P2RX7_HUMAN antibody
    • P2X purinoceptor 7 antibody
    • P2X7 antibody
    • P2Z receptor antibody
    • Purinergic receptor antibody
    • purinergic receptor P2X, ligand gated ion channel, 7 antibody
    see all

Anti-P2RX7 antibody 图像

  • All lanes : Anti-P2RX7 antibody (ab93354) at 0.3 µg/ml

    Lane 1 : Human brain frontal cortex lysate
    Lane 2 : Human brain frontal cortex lysate with Immunising peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 37,70 kDa (why is the actual band size different from the predicted?)
  • ab87597 at 3.8 µg/ml staining P2RX7 in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

Anti-P2RX7 antibody (ab93354)参考文献

ab93354 has not yet been referenced specifically in any publications.

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