Rat, Cow, Dog
Synthetic peptide with sequence C-YREKKYKYVEDYEQ, from the C terminus of the human P2RX4 protein (NP_002551.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
ab77434 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Belongs to the P2X receptor family.
Information by UniProt
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
Western blot - Anti-P2RX4 antibody (ab77434)
All lanes :
Anti-P2RX4 antibody (ab77434) at 1 µg/mlLane 1 :
Human kidney lysate (in RIPA buffer)Lane 2 :
Human liver lysate (in RIPA buffer)Lane 3 :
Rat kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.Predicted band size:
43 kDaObserved band size:
60 kDa (why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Detected by chemiluminescence.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"