概述

  • 产品名称
  • 描述
    兔多克隆抗体to P2RX4
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human P2RX4.

    (Peptide available as ab89385.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Brain Tissue; SHSY-5Y Whole Cell; SK N BE Whole Cell; SK N SH Whole Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab76893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能
    Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
  • 序列相似性
    Belongs to the P2X receptor family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI504491 antibody
    • ATP gated cation channel protein antibody
    • ATP receptor antibody
    • AW555605 antibody
    • D5Ertd444e antibody
    • fi03f02 antibody
    • MGC143034 antibody
    • OTTMUSP00000023741 antibody
    • OTTMUSP00000023745 antibody
    • OTTMUSP00000023746 antibody
    • OTTMUSP00000023747 antibody
    • P2rx4 antibody
    • P2RX4_HUMAN antibody
    • P2X purinoceptor 4 antibody
    • P2X receptor subunit 4 antibody
    • P2X4 antibody
    • P2X4R antibody
    • Purinergic receptor antibody
    • Purinergic receptor P2X ligand gated ion channel 4 antibody
    • Purinergic receptor P2X4 antibody
    • Purinoceptor P2X4 antibody
    • wu:fi03f02 antibody
    see all

图片

  • All lanes : Anti-P2RX4 antibody (ab76893) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

实验方案

文献

ab76893 has not yet been referenced specifically in any publications.

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