All lanes : Anti-p27 KIP 1 (phospho T157) antibody (ab85047) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human p27 KIP 1 (phospho T157) peptide (
ab97604) at 1 µg/ml
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human p27 KIP 1 (phospho T157) peptide (
ab97604) at 1 µg/ml
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Human p27 KIP 1 (phospho T157) peptide (
ab97604) at 1 µg/ml
Lane 8 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Human p27 KIP 1 (phospho T157) peptide (
ab97604) at 1 µg/ml
Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with p27 KIP 1 peptide (
ab116623) at 1 µg/ml
Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with p27 KIP 1 peptide (
ab116623) at 1 µg/ml
Lane 11 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with p27 KIP 1 peptide (
ab116623) at 1 µg/ml
Lane 12 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with p27 KIP 1 peptide (
ab116623) at 1 µg/ml
SecondaryAll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 32 kDa (
why is the actual band size different from the predicted?)
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
The 32kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human Cyclin-dependent kinase inhibitor 1B (p27 KIP 1).