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A synthetic phosphopeptide corresponding to residues surrounding Serine 10 of human p27 KIP 1.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab62364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/20000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/200 - 1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde fixed paraffin-embedded monkey cerebellum sections. Antigen retrieval step: Heat mediated. Buffer: Citric acid solution. Permeabilization: None. Blocking step: 1% BSA for 10 mins. Primary antibody incubated at 1/1500 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody Name: anti rabbit IgG conjugated to biotin (1/200).
Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde-fixed paraffin-embedded human tonsil sections. Antigen retrieval step: Heat mediated. Buffer Used: Citric acid. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/500 for 2 hours @ 21°C in TBS/BSA/azide. Notes: A micropolymer HRP kit was used to obtain this intensity of immunostaining as I could not achieve a similar intensity of positivity on human tissues, under the same conditions (compare with results on rat/mouse/marmoset sections, using a std ABCpx detection system). There is one obvious metaphase nucleus in the Germinal centre of the submitted image: it is not positive.
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