概述

  • 产品名称Anti-p27 KIP 1抗体[EPFHCR16]
    参阅全部 p27 KIP 1 一抗
  • 描述
    兔单克隆抗体[EPFHCR16] to p27 KIP 1
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    A recombinant protein within Mouse p27 KIP 1.

  • 阳性对照
    • NIH3T3, C6 and Neuro 2a cell lysates; Mouse lung and thymus tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/1000 - 1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).

    Is unsuitable for Flow Cyt, ICC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
    • 组织特异性Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
    • 疾病相关Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
    • 序列相似性Belongs to the CDI family.
    • 结构域A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.
    • 翻译后修饰Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
      Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
      Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.
    • 细胞定位Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AA408329 antibody
      • AI843786 antibody
      • Cdki1b antibody
      • CDKN 1B antibody
      • CDKN 4 antibody
      • CDKN1B antibody
      • CDKN4 antibody
      • CDN1B_HUMAN antibody
      • Cyclin Dependent Kinase Inhibitor 1B antibody
      • Cyclin dependent kinase inhibitor p27 antibody
      • Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody
      • Cyclin-dependent kinase inhibitor 1B antibody
      • Cyclin-dependent kinase inhibitor p27 antibody
      • Cyclin-dependent kinase inhibitor p27 Kip1 antibody
      • KIP 1 antibody
      • KIP1 antibody
      • MEN1B antibody
      • MEN4 antibody
      • OTTHUMP00000195098 antibody
      • OTTHUMP00000195099 antibody
      • p27 antibody
      • p27 Kip1 antibody
      • P27-like cyclin-dependent kinase inhibitor antibody
      • p27Kip1 antibody
      see all

    Anti-p27 KIP 1 antibody [EPFHCR16] 图像

    • All lanes : Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Neuro 2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    • ab92741 at 1/100 dilution staining p27 KIP in Mouse lung by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92741 at 1/100 dilution staining p27 KIP in Mouse thymus by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)参考文献

    This product has been referenced in:
    • Ding Z  et al. SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression. Nature 470:269-73 (2011). Mouse . Read more (PubMed: 21289624) »

    See 1 Publication for this product

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