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Our Abpromise guarantee covers the use of ab3928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/50 - 1/100. Predicted molecular weight: 22 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24658003|
ab3928 at a 1/200 dilution staining rat brain (dentate gyrus, sub-ventricular zone) tissue sections by Immunohistochemistry (Frozen sections). The antibody was added to the tissue sections for 24 hours and then detected using an Alexa Fluor ® 488 conjugated rabbit anti-mouse antibody.
This image is courtesy of an Abreview.