Anti-p23抗体(ab226295)
Key features and details
- Rabbit polyclonal to p23
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-p23抗体
参阅全部 p23 一抗 -
描述
兔多克隆抗体to p23 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Orangutan -
免疫原
Synthetic peptide within Human p23 aa 110-160. The exact sequence is proprietary. (NP_006592.3).
Database link: Q15185 -
阳性对照
- IHC-P: Human lung cancer and ovarian carcinoma tissues. Mouse teratoma tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab226295 was affinity purified using an epitope specific to p23 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226295于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/1000. Predicted molecular weight: 19 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/2000 - 1/10000.
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说明 |
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WB
1/2000 - 1/1000. Predicted molecular weight: 19 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/2000 - 1/10000. |
靶标
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功能
Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the p23/wos2 family.
Contains 1 CS domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 396544 Chicken
- Entrez Gene: 493638 Cow
- Entrez Gene: 10728 Human
- Entrez Gene: 100043508 Mouse
- Entrez Gene: 100048119 Mouse
- Entrez Gene: 56351 Mouse
- Entrez Gene: 100174118 Orangutan
- Entrez Gene: 362809 Rat
see all -
别名
- Co chaperone p23 antibody
- cPGES antibody
- Cytosolic prostaglandin E synthase antibody
see all
图片
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All lanes : Anti-p23 antibody (ab226295) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for p23 using ab226295 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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p23 was immunoprecipitated from HEK-293T (human epithelial epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226295 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226295 at 1 µg/ml.
Lane 1: ab226295 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Cells prepared using NETN lysis buffer.
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for p23 using ab226295 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for p23 using ab226295 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226295 尚未被引用在任何文献中。