Anti-p21-ARC抗体(ab96137)

概述

  • 产品名称
    Anti-p21-ARC抗体
    参阅全部 p21-ARC 一抗
  • 描述
    兔多克隆抗体to p21-ARC
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Zebrafish
  • 免疫原

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 165 of Human p21-ARC (NP_005710).

  • 阳性对照
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and cell lines. Raji whole cell lysate for WB and endo mitral OVCA tissue for IHC.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).
  • 细胞定位
    Cytoplasmic; cytoskeleton
  • 数据库链接
  • 别名
    • Actin related protein 2/3 complex subunit 3 antibody
    • ARC21 antibody
    • ARP2/3 complex 21 kDa subunit antibody
    • ARP2/3 protein complex subunit p21 antibody
    • ARPC3 antibody
    see all

图片

  • Anti-p21-ARC antibody (ab96137) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • ab96137, at 1/100, staining paraffin-embedded endo mitral OVCA by Immunohistochemistry.

文献

ab96137 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell line)
Loading amount
50 µg
Specification
Breast cancer cell line
Gel Running Conditions
Reduced Denaturing (12% and 15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Otto Cerqueira

Verified customer

提交于 Oct 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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