概述

  • 产品名称
    Anti-p21-ARC抗体
    参阅全部 p21-ARC 一抗
  • 描述
    山羊多克隆抗体to p21-ARC
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-RQFMNKSLSGPGQ

    , corresponding to C terminal amino acids 165-177 of Human p21-ARC

  • 阳性对照
    • WB: Hela, Human Brain and Mouse Brain lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

靶标

  • 相关性
    p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).
  • 细胞定位
    Cytoplasmic; cytoskeleton
  • 数据库链接
  • 别名
    • Actin related protein 2/3 complex subunit 3 antibody
    • ARC21 antibody
    • ARP2/3 complex 21 kDa subunit antibody
    • ARP2/3 protein complex subunit p21 antibody
    • ARPC3 antibody
    see all

Anti-p21-ARC antibody 图像

  • Anti-p21-ARC antibody (ab37916) at 1 µg/ml + Mouse Brain lysate (RIPA buffer 35µg total protein per lane).

    Predicted band size : 20 kDa

实验方案

Anti-p21-ARC antibody (ab37916)参考文献

This product has been referenced in:
  • Lippi G  et al. Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology. J Cell Biol 194:889-904 (2011). WB ; Mouse . Read more (PubMed: 21930776) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Zebrafish Cell lysate - whole cell (whole larval cell lysate 48-72 hours post fertiliz)
Specification
whole larval cell lysate 48-72 hours post fertiliz
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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