Anti-p21抗体[HUGO291] (ab107099)

概述

  • 产品名称
    Anti-p21抗体[HUGO291]
    参阅全部 p21 一抗
  • 描述
    大鼠单克隆抗体[HUGO291] to p21
  • 特异性
    This antibody is not suitable for use in human.
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Immunogen information is not available

  • 阳性对照
    • Mouse skin papilloma.

性能

应用

Our Abpromise guarantee covers the use of ab107099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 18 kDa.
ELISA 1/1000.
IHC-P 1/100.

靶标

  • 功能
    May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
  • 组织特异性
    Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.
  • 序列相似性
    Belongs to the CDI family.
  • 结构域
    The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
  • 翻译后修饰
    Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP20 antibody
    • CDK-interacting protein 1 antibody
    • CDKI antibody
    • CDKN1 antibody
    • Cdkn1a antibody
    • CDN1A_HUMAN antibody
    • CIP1 antibody
    • Cyclin Dependent Kinase Inhibitor 1A antibody
    • Cyclin-dependent kinase inhibitor 1 antibody
    • Cyclin-dependent kinase inhibitor 1A (P21) antibody
    • Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody
    • DNA Synthesis Inhibitor antibody
    • MDA-6 antibody
    • MDA6 antibody
    • Melanoma differentiation-associated protein 6 antibody
    • Melanoma differentiation-associated protein antibody
    • p21 antibody
    • P21 protein antibody
    • p21CIP1 antibody
    • p21Cip1/Waf1 antibody
    • p21WAF antibody
    • PIC1 antibody
    • SDI1 antibody
    • SLC12A9 antibody
    • WAF1 antibody
    • Wild type p53 activated fragment 1 (WAF1) antibody
    • Wild type p53 activated fragment 1 antibody
    • Wildtype p53-activated fragment 1 antibody
    see all

图片

  • All lanes : Anti-p21 antibody [HUGO291] (ab107099) at 1/50 dilution

    Lane 1 : TT243 MEFs (non-irradiated) at 18.83 µg
    Lane 2 : TT243 MEFs (irradiated with 10 Gy) at 20.8 µg
    Lane 3 : p21-/- KO MEFs at 60 µg
    Lane 4 : P21 WT MEFs at 75 µg
    Lane 5 : HEK-V5-mp21 transfected cells at 20 µg
    Lane 6 : HEK-V5-mp15 transfected cells at 20 µg


    Predicted band size : 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse p21WT fibrosarcoma and mouse p21KO fibrosarcoma tissue sections labeling p21 with ab107099.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hek-V5-mp21 transfected cells labelling p21 with ab107099. Nuclear staining was observed in Hek-V5-mp21 cytospins. Labeling with the anti-V5 mAb confirmed the efficiency of transfection. Cytospin preparation of human CD8 was used as a negative control.

  • ab107099 at 1/100 staining p21 in conditional TRF1 knock out Mouse skin tissue by Immunohistochemistry.

  • ab107099 at 1/100 staining p21 in Mouse skin papilloma tissue by Immunohistochemistry.

文献

ab107099 has not yet been referenced specifically in any publications.

客户评价及客户问答


Yesterday we spoke of the recommended WB dilution for ab107099 listed as 1/2 on the datasheet. I spoke with the lab, and they responded that with the purified form of the antibody the recommended WB dilution is 1/50. The dilutions used in the imag...

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