Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (13%)
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblast (MEF) cell lysates)
Specification
mouse embryonic fibroblast (MEF) cell lysates
Treatment
DMSO control (Ctl) and 7,5 ´M CPT-11 (CPT) for 24 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in PBS-T buffer
Dilution
1/2500
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)
Detection
Detection method
ECL
Bands
Specific: 16 kDa Non-specific: 14, 30, 55 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
G2 phase cell cycle arrest induced by CPT-11 (Irinotecan) treatment in MEF cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christian Marx
Verified customer
提交于 Dec 02 2013