Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MEF
Blocking step
ODYSSEY BLOCKING BUFFER as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Donkey ANTI RABIT IRDye 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 680
Detection
Detection method
Li-Cor
Exposure
25 minute(s) and 0 second(s)
Positive control
tubulin
Additional data
Additional Notes
The membrane was blotted agains anti tubulin (ab6046) and anti p21 (ab109520).
Abcam response
Thank you so much for testing this antibody in mouse. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species.
The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Muhammed Zoabi
Verified customer
提交于 Apr 27 2017