概述

  • 产品名称Anti-p19 INK4d抗体
    参阅全部 p19 INK4d 一抗
  • 描述
    小鼠单克隆抗体to p19 INK4d
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human) with a 26kDa tag

  • 阳性对照
    • p19 INK4D transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 相关性Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • CDK inhibitor p19INK4d antibody
    • CDKN2D antibody
    • cell cycle inhibitor, Nur77 associating protein antibody
    • Cyclin dependent kinase inhibitor 2D antibody
    • Cyclin-dependent kinase 4 inhibitor D antibody
    • Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) antibody
    • inhibitor of cyclin-dependent kinase 4d antibody
    • INK4D antibody
    • MGC109660 antibody
    • MGC137547 antibody
    • MGC73010 antibody
    • p19 antibody
    • p19-INK4D antibody
    • p19INK4d antibody
    • Similar to cyclin-dependent kinase inhibitor 2D antibody
    see all

Anti-p19 INK4d antibody 图像

  • Anti-p19 INK4d antibody (ab77223) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-p19 INK4d antibody (ab77223) at 5 µg/ml

    Lane 1 : p19 INK4d-transfected 293T cell lysate
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

Anti-p19 INK4d antibody (ab77223)参考文献

ab77223 has not yet been referenced specifically in any publications.

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