概述

  • 产品名称
    Anti-p16 ARC抗体
    参阅全部 p16 ARC 一抗
  • 描述
    兔多克隆抗体to p16 ARC
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human p16 ARC.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: TE671; HT1080; U20S; HL60; HeLa; HT29. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MCF-7.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).

靶标

图片



  • Predicted band size : 16 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: p16 ARC knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HT29 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab118459 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab118459 was shown to recognize p16 ARC when p16 ARC knockout samples were used, along with additional cross-reactive bands. Wild-type and p16 ARC knockout samples were subjected to SDS-PAGE.  Ab118459 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • IHC image of p16 ARC staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab118459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab118459 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab118459 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa, Hek293, and HepG2 cell types, and in Methanol fixed (100%, 5min) HeLa, and HepG2 cell types.

  • All lanes : Anti-p16 ARC antibody (ab118459) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

文献

This product has been referenced in:
  • Qiu M  et al. MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells. Exp Ther Med 13:1329-1336 (2017). WB ; Human . Read more (PubMed: 28413473) »
  • Kahr WH  et al. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nat Commun 8:14816 (2017). Read more (PubMed: 28368018) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for contacting us.

I am assuming the species customer of tissue sections is human - antibodeis are asfollows;

The best anti annexin a1 antibody is ab47661;http://www.abcam.com/Annexin-A1-antibody-ab47661.html

anti I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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