Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse lung)
Specification
mouse lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: ~16 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
Lane 1: Protein Molecular Weight Marker (Bio-Rad)
Lanes 2-5: Mouse lung homogenate (RIPA buffer was used to prepare lysates)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 27 2015