Anti-p150 CAF1抗体[1SS-48] (ab30327)

概述

  • 产品名称Anti-p150 CAF1抗体[1SS-48]
    参阅全部 p150 CAF1 一抗
  • 描述
    小鼠单克隆抗体[1SS-48] to p150 CAF1
  • 特异性ab30327 recognises Chromatin Assembly Factor 1 (CAF-1).
  • 经测试应用适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Human p150 CAF1 subunit protein.

性能

应用

Our Abpromise guarantee covers the use of ab30327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IP
WB
  • 应用说明IF: Use at a concentration of 2.5 µg/ml.
    IP: Use at a concentration of 2.5 µg/sample.
    WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 105 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • 序列相似性Belongs to the CHAF1A family.
    • 发展阶段Active complex is found in G1, S and G2 phases.
    • 结构域Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. DNA replication foci.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CAF 1 antibody
      • CAF 1 subunit A antibody
      • CAF antibody
      • CAF I 150 kDa subunit antibody
      • CAF I p150 antibody
      • CAF-1 subunit A antibody
      • CAF-I 150 kDa subunit antibody
      • CAF-I p150 antibody
      • CAF1 antibody
      • CAF1A_HUMAN antibody
      • CAF1B antibody
      • CAF1P150 antibody
      • CHAF1A antibody
      • Chromatin assembly factor 1 subunit A antibody
      • Chromatin Assembly Factor 1 Subunit A p150 antibody
      • Chromatin assembly factor I (150 kDa) antibody
      • Chromatin assembly factor I 150 kDa antibody
      • Chromatin assembly factor I p150 subunit antibody
      • DCAF1 antibody
      • hp150 antibody
      • MGC71229 antibody
      • P150 antibody
      see all

    Anti-p150 CAF1 antibody [1SS-48] (ab30327)参考文献

    ab30327 has not yet been referenced specifically in any publications.

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