Anti-p15 INK4b抗体(ab94688)


  • 产品名称Anti-p15 INK4b抗体
    参阅全部 p15 INK4b 一抗
  • 描述
    兔多克隆抗体to p15 INK4b
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human p15 INK4b.

    (Peptide available as ab109892.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; A549; HepG2; Caco 2. IHC-P: Human colon FFPE tissue sections



Our Abpromise guarantee covers the use of ab94688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
  • 序列相似性Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDK inhibitory protein antibody
    • CDK4B Inhibitor antibody
    • Cdkn2b antibody
    • CDN2B_HUMAN antibody
    • Cyclin dependent kinase 4 inhibitor B antibody
    • Cyclin Dependent Kinase Inhibitor 2B antibody
    • Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4 antibody
    • Cyclin dependent kinases 4 and 6 binding protein antibody
    • Cyclin-dependent kinase 4 inhibitor B antibody
    • INK4B antibody
    • MTS 2 antibody
    • MTS-2 antibody
    • MTS2 antibody
    • Multiple tumor suppressor 2 antibody
    • Multiple Tumor Supressor 2 antibody
    • OTTHUMP00000021154 antibody
    • OTTHUMP00000021155 antibody
    • p14 CDK inhibitor antibody
    • p14 INK4b antibody
    • p14-INK4b antibody
    • P15 antibody
    • p15 CDK inhibitor antibody
    • p15 inhibits CDK4 antibody
    • p15 INK4b antibody
    • p15-INK4b antibody
    • p15INK4B antibody
    • TP 15 antibody
    • TP15 antibody
    see all

Anti-p15 INK4b antibody 图像

  • IHC image of p15 INK4b staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94688, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-p15 INK4b antibody (ab94688) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab94688 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94688, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab94688, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and MCF7 cells at 5µg/ml.

Anti-p15 INK4b antibody (ab94688)参考文献

ab94688 has not yet been referenced specifically in any publications.

Product Wall

This protein is known to be both nuclear and cytoplasmic (

Our lab tested this antibody in ICC/IF and passed this antibody based on cytoplasmic localisation data. The image legend ...

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