RabMAb

Anti-p130 (phospho S672)抗体[EP2126Y] (ab76255)

概述

  • 产品名称Anti-p130 (phospho S672)抗体[EP2126Y]
    参阅全部 p130 一抗
  • 描述
    兔单克隆抗体[EP2126Y] to p130 (phospho S672)
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues surrounding Serine 672 of human p130

  • 阳性对照
    • Jurkat cell lysates, treated or untreated with AP.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
IP 1/20.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
    • 序列相似性Belongs to the retinoblastoma protein (RB) family.
    • 发展阶段G0-restricted expression.
    • 翻译后修饰During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 130 kDa retinoblastoma associated protein antibody
      • 130 kDa retinoblastoma-associated protein antibody
      • IB4 antibody
      • p130 antibody
      • PPAR-alpha-interacting complex protein 128 antibody
      • PRB2 antibody
      • Rb2 antibody
      • Rbl2 antibody
      • RBL2_HUMAN antibody
      • RBR 2 antibody
      • RBR-2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma-like protein 2 antibody
      • Retinoblastoma-related protein 2 antibody
      • RIC128 antibody
      see all

    Anti-p130 (phospho S672) antibody [EP2126Y] 图像

    • All lanes : Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution

      Lane 1 : Jurkat cell lysates, treated with AP
      Lane 2 : Jurkat cell lysates, untreated

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 128 kDa
      Observed band size : 128 kDa

    Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255)参考文献

    This product has been referenced in:
    • Bohrer LR  et al. Androgens suppress EZH2 expression via retinoblastoma (RB) and p130-dependent pathways: a potential mechanism of androgen-refractory progression of prostate cancer. Endocrinology 151:5136-45 (2010). WB ; Human . Read more (PubMed: 20881251) »

    See 1 Publication for this product

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