RabMAb

Anti-p130抗体[EP2141Y] (ab76234)

概述

  • 产品名称Anti-p130抗体[EP2141Y]
    参阅全部 p130 一抗
  • 描述
    兔单克隆抗体[EP2141Y] to p130
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p130 aa 1100 to the C-terminus (C terminal).

  • 阳性对照
    • WB: Jurkat and Daudi cell lysates. IHC-P: Human breast carcinoma and liver tissues, rat kidney and mouse liver tissues. Flow Cyt: Jurkat cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab76234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
IHC-P 1/250 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC/IF or IP.
  • 靶标

    • 功能Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
    • 序列相似性Belongs to the retinoblastoma protein (RB) family.
    • 发展阶段G0-restricted expression.
    • 翻译后修饰During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 130 kDa retinoblastoma associated protein antibody
      • 130 kDa retinoblastoma-associated protein antibody
      • IB4 antibody
      • p130 antibody
      • PPAR-alpha-interacting complex protein 128 antibody
      • PRB2 antibody
      • Rb2 antibody
      • Rbl2 antibody
      • RBL2_HUMAN antibody
      • RBR 2 antibody
      • RBR-2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma-like protein 2 antibody
      • Retinoblastoma-related protein 2 antibody
      • RIC128 antibody
      see all

    Anti-p130 antibody [EP2141Y] 图像

    • Anti-p130 antibody [EP2141Y] (ab76234) at 1/1000 dilution (unpurified) + Daudi cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 128 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-p130 antibody [EP2141Y] (ab76234) at 1/1000 dilution (purified) + Daudi cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 128 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labelling p130 with unpurified ab76234 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labelling p130 with purified ab76234 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling p130 with unpurified ab76234 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling p130 with purified ab76234 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labelling p130 with unpurified ab76234 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labelling p130 with purified ab76234 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling p130 with unpurified ab76234 at 1/50.

    • Overlay histogram showing Jurkat cells stained with unpurified ab76234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76234, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-p130 antibody [EP2141Y] (ab76234)参考文献

    ab76234 has not yet been referenced specifically in any publications.

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