Recombinant fragment: VTPVSTATHS LSRLHTMLTG LRNAPSEKLE QILRTCSRDP TQAIANRLKE MFEIYSQHFQ PDEDFSNCAK EIASKHFRFA EMLYYKVLES VIEQEQKRLG , corresponding to amino acids 416-516 of Human p130 with proprietary tag (NP_005602)
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
Use at an assay dependent dilution.
相关性p130 is a key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.