Customer reviews and FAQs

概述

  • 产品名称Anti-p114RhoGEF抗体
    参阅全部 p114RhoGEF 一抗
  • 描述
    兔多克隆抗体to p114RhoGEF
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 612-802 of Human ARHGEF18 (NP_056133)

  • 阳性对照
    • HepG2 whole cell lysate; 293T, A431 and H1299 cells This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: Isoform 1, 131 ; Isoform 2, 114 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • Information by UniProt
  • 数据库链接
    • 别名
      • 114 kDa Rho specific guanine nucleotide exchange factor antibody
      • 114 kDa Rho-specific guanine nucleotide exchange factor antibody
      • ARHGEF 18 antibody
      • Arhgef18 antibody
      • ARHGI_HUMAN antibody
      • KIAA0521 antibody
      • MGC15913 antibody
      • p114 Rho GEF antibody
      • p114-Rho-GEF antibody
      • p114RhoGEF antibody
      • Rho guanine nucleotide exchange factor 18 antibody
      • Rho specific guanine nucleotide exchange factor p114 antibody
      • Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody
      • Rho/Rac guanine nucleotide exchange factor 18 antibody
      • SA RhoGEF antibody
      • SA-RhoGEF antibody
      • Septin associated RhoGEF antibody
      • Septin-associated RhoGEF antibody
      see all

    Anti-p114RhoGEF antibody 图像

    • Immunocytochemistry/ Immunofluorescence - Anti-p114RhoGEF antibody (ab96520)
      Immunocytochemistry/ Immunofluorescence - Anti-p114RhoGEF antibody (ab96520)

      ICC/IF image of ab96520 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96520 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Western blot - p114RhoGEF antibody (ab96520)
      Western blot - p114RhoGEF antibody (ab96520)
      Anti-p114RhoGEF antibody (ab96520) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size : Isoform 1, 131 ; Isoform 2, 114 kDa
      7.5% SDS-PAGE

    Anti-p114RhoGEF antibody (ab96520)参考文献

    This product has been referenced in:
    • Hu J  et al. MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1. PLoS One 8:e68291 (2013). WB ; Human . Read more (PubMed: 23826386) »

    See 1 Publication for this product

    发表研究结果有使用 ab96520？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab96520 的 abreview 提交 ab96520 的问题

    There are currently no Abreviews or Questions for ab96520.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"