概述

  • 产品名称
    Anti-P Glycoprotein抗体
    参阅全部 P Glycoprotein 一抗
  • 描述
    兔多克隆抗体to P Glycoprotein
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, African green monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 863-912 of Human P Glycoprotein (NP_000918).

  • 阳性对照
    • NCI-H226 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab98322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • 组织特异性
    Expressed in liver, kidney, small intestine and brain.
  • 疾病相关
    Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • 序列相似性
    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

图片

  • Anti-P Glycoprotein antibody (ab98322) at 1 µg/ml (in 5% skim milk / PBS buffer) + NCI-H226 cell lysate at 10 µg

    Predicted band size : 141 kDa
  • ab98322 staining P Glycoprotein in placental tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab98322 staining P Glycoprotein in adrenal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

文献

This product has been referenced in:
  • Yoshimori M  et al. P-glycoprotein is expressed and causes resistance to chemotherapy in EBV-positive T-cell lymphoproliferative diseases. Cancer Med 4:1494-504 (2015). IHC . Read more (PubMed: 26153782) »
  • Ma X  et al. Essential role for TrpC5-containing extracellular vesicles in breast cancer with chemotherapeutic resistance. Proc Natl Acad Sci U S A 111:6389-94 (2014). ICC/IF ; Human . Read more (PubMed: 24733904) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for passing on that reply.

I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

As I mentioned in my previous email, I would be happy to provide the ...

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Thank you for your kinds words. I am happy to help!

As requested, I have issued a free of charge replacement for one vial of ab27671.

To check the status of the order please contact our Customer Service team and reference this numbe...

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Thanks for your reply. I am sorry to hear the replacement vial of ab3366 did not improve things.

Just to make sure, you would like me to send along one vial of ab27671 as a replacement for this most recent faulty vial of ab3366? If this is yo...

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I'm happy to help! Please let me know if you need anything further.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab3366.

To check the status of the order please contact our...

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Thanks so much for sending along your blot and for the additional protocol details.

I notice there are 8 different samples on the blot. Do you expect any variation in MDR1 expression between these samples?

Thanksfor the additional i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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