Anti-P Glycoprotein抗体[4E3] (ab10333)

概述

  • 产品名称Anti-P Glycoprotein抗体[4E3]
    参阅全部 P Glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[4E3] to P Glycoprotein
  • 特异性P-glycoprotein - 170 Kda (External Epitope) The 4E3 monoclonal antibody recognizes an epitope of the mdr1 encoded P-glycoprotein located on the exterior surface of the plasma membrane. 4E3 does not cross-react with the mdr3 gene product.
  • 经测试应用适用于: ICC, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human squamous lung cancer cell line SW-1573/500).

  • 阳性对照
    • Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue: Liver (positive staining detected along luminal surfaces of bile canaliculi) or colon (positive staining localized to luminal surface of secretory epithelium).
  • 常规说明


    P-glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias/lymphomas.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明P-glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias/lymphomas.
  • 克隆单克隆
  • 克隆编号4E3
  • 同种型IgG2a
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab10333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/10.
Flow Cyt 1/10. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • 组织特异性Expressed in liver, kidney, small intestine and brain.
  • 疾病相关Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

Anti-P Glycoprotein antibody [4E3] (ab10333)参考文献

This product has been referenced in:
  • Dessilly G  et al. ABCB1 1199G>A genetic polymorphism (Rs2229109) influences the intracellular accumulation of tacrolimus in HEK293 and K562 recombinant cell lines. PLoS One 9:e91555 (2014). ICC/IF ; Human . Read more (PubMed: 24621983) »
  • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . Read more (PubMed: 22634564) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.


I heard back from the supplying lab that theyhave not mapped the exact epitope for this antibody. We have licensed this from the inventor of the antibody. The only information we have about it was provided by...

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Thank you for your phone call.
As we discussed, ab10333 has not been validated for use in WB and may or may not be suitable for use in that application. While it will not be covered under our Abpromise guarantee, the following may help to improve ...

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Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (liver, hepatoma, HepG2)
Specification liver, hepatoma, HepG2
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Tx100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
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Verified customer

提交于 Aug 31 2007

Thank you for your email. I do apologize as I think that a reply got lost somehow in the system. For this antibody, we were selling it in a smaller size (100 ul) but it did not provide enough tests. We are now providing it in a 500 ul size. If your...

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Thank you for your enquiry. We have received the following information from the source of ab0333: "We recommend that the customer start with a 1:10 dilution and titer Ab from there for their individual application." In light of this informatio...

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Thank you for your enquiry and for your patience. Unfortunately, none of these antibodies are available in a conjugated form but we do have a wide range of secondary antibodies to choose from (including ones conjugated to FITC and PE). At the bottom of...

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Thank you for your enquiry regarding ab10333. I recommend you refer to our general flow cytometry protocol for all relevant flow cytometry information. You can view this information by clicking on the protocol tab on our online product datasheet. If yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"