概述

  • 产品名称Anti-P cadherin抗体[NCC-CAD-299]
    参阅全部 P cadherin 一抗
  • 描述
    小鼠单克隆抗体[NCC-CAD-299] to P cadherin
  • 特异性This antibody specifically reacts with human P Cadherin, and inhibits P Cadherin dependent cell-cell contact.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, WB, Inhibition Assaymore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human epidermal carcinoma cell line A431.

性能

应用

Our Abpromise guarantee covers the use of ab12222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 91.4 kDa.
Inhibition Assay Use a concentration of 100 µg/ml.

靶标

  • 功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • 组织特异性Expressed in some normal epithelial tissues and in some carcinoma cell lines.
  • 疾病相关Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • 序列相似性Contains 5 cadherin domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Anti-P cadherin antibody [NCC-CAD-299] 图像

  • Overlay histogram showing A431 cells stained with ab12222 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12222, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-P cadherin antibody [NCC-CAD-299] (ab12222)参考文献

This product has been referenced in:
  • Chanson L  et al. Self-organization is a dynamic and lineage-intrinsic property of mammary epithelial cells. Proc Natl Acad Sci U S A 108:3264-9 (2011). Inhibition Assay ; Human . Read more (PubMed: 21300877) »

See 1 Publication for this product

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