Anti-P cadherin抗体[MM0508-9V11] (ab89900)

概述

  • 产品名称Anti-P cadherin抗体[MM0508-9V11]
    参阅全部 P cadherin 一抗
  • 描述
    小鼠单克隆抗体[MM0508-9V11] to P cadherin
  • 经测试应用适用于: WB, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human recombinant P cadherin extracellular domain.

性能

  • 形式Lyophilised:Reconstitute the antibody with 200µl sterile PBS and the final concentration is 0.5mg/ml.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明filter sterilised
  • 克隆单克隆
  • 克隆编号MM0508-9V11
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • 组织特异性Expressed in some normal epithelial tissues and in some carcinoma cell lines.
  • 疾病相关Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • 序列相似性Contains 5 cadherin domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Anti-P cadherin antibody [MM0508-9V11] 图像

  • Overlay histogram showing A431 cells stained with ab89900 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89900, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-P cadherin antibody [MM0508-9V11] (ab89900)参考文献

ab89900 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (Human embryonic stem cells)
Permeabilization No
Gating Strategy Isotype control
Specification Human embryonic stem cells
Fixation unfixed
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Verified customer

提交于 Jun 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human embryonic stem cells)
Permeabilization Yes - 0.1% Triton
Specification Human embryonic stem cells
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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Verified customer

提交于 May 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"