Anti-Oxytocin Receptor抗体(ab87312)

Abreviews (1)Q&A (14)Specific References (1)

概述

  • 产品名称
    Anti-Oxytocin Receptor抗体
    参阅全部 Oxytocin Receptor 一抗
  • 描述
    山羊多克隆抗体to Oxytocin Receptor
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Oxytocin Receptor aa 355-367 (C terminal).
    Sequence:

    C-RRLGETSASKKSN


    Database link: P30559
    (Peptide available as ab173096)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

应用

Our Abpromise guarantee covers the use of ab87312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Oxytocin Receptor peptide (ab173096).

Approx 75kDa band observed in Rat Uterus lysates, but none in Rat Skeletal Muscle lysates as expected (calculated MW of 42.9kDa according to Rat NP_037003.2.

ELISA Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1:4000
ICC/IF Use a concentration of 5 - 10 µg/ml.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr 1/150.

靶标

图片

  • Immunocytochemistry/ Immunofluorescence-Anti-Oxytocin Receptor antibody(ab87312)
    Immunocytochemistry/ Immunofluorescence-Anti-Oxytocin Receptor antibody(ab87312)
    ab87312 staining oxytocin receptor in MCF7 cells treated with oxytocin (ab120186), by ICC/IF. Increase of cytoplasmatic oxytocin receptor localization and correspondent decrease in nuclear oxytocin receptor correlates with increased concentration of oxytocin, as described in literature.
    The cells were incubated at 37°C for 15 minutes in media containing different concentrations of ab120186 (oxytocin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab87312 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Immunocytochemistry/ Immunofluorescence - Oxytocin Receptor antibody (ab87312)
    Immunocytochemistry/ Immunofluorescence - Oxytocin Receptor antibody (ab87312)
    ab87312 at 5 µg/ml staining Oxytocin Receptor in Human prostate stromal cells by Immunocytochemistry/ Immunofluorescense. Antibody was incubated overnight at 4°C and detected with an AlexaFluor-conjugated secondary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin Receptor antibody (ab87312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin Receptor antibody (ab87312)

    Immunohistochemical analysis of paraffin embedded Human prostatic cancer tissue labeling Oxytocin Receptor with ab87312 at 2 ug/ml.

  • Immunohistochemistry (Frozen sections) - Anti-Oxytocin Receptor antibody (ab87312)
    Immunohistochemistry (Frozen sections) - Anti-Oxytocin Receptor antibody (ab87312)
    ab87312 staining Oxytocin Receptor in rat brain hypothalamus tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 1 hour at 25°C and then incubated with ab87312 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated donkey anti-goat IgG polyclonal used at a 1/1000 dilution.

    Magnocellular hypothalamus was stained.

    See Abreview

文献

This product has been referenced in:
  • Gong L  et al. The antinociception of oxytocin on colonic hypersensitivity in rats was mediated by inhibition of mast cell degranulation via Ca(2+)-NOS pathway. Sci Rep 6:31452 (2016). IF . Read more (PubMed: 27538454) »

See 1 Publication for this product

发表研究结果有使用 ab87312？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

15 reviews or Q&A

10
Votes: Highest First

Can you tell me the molecular weight of the blocking peptide for the Anti-Oxytocin Receptor antibody?

The sequence of the peptide is C-RRLGETSASKKSN with a calculated MW of 1536.73 Daltons.

I could not find a blocking peptide for ab87312 in your catalog. Do you guys manufacture it?

I have asked our product managers to add the blocking peptide for ab87312 Anti-Oxytocin Receptor antibody. This peptide can now be found as ab173096 - Oxytocin Receptor peptide, at the following location: http://www.abcam.com/oxytocin-receptor-pepti...

Read More

Can you inform whether you or your customer has tested this Ab positive in mouse brain? If yes, can you inform which anatomy location of the mouse brain is positive? And related literature reference will be appreciated. We want to try it again.

Thank you for your reply.

We have not had any feedback from customers using this antibody on mouse samples. The image on our datasheet shows staining in rat hypothalamus. You can also view the expected expression of Oxytocin receptor in mouse...

Read More

Does ab87312 reacts with mouse tissue? The datasheet says it only reacts with rat and human. The reason that it didn't work, could be the Ab doesn't recognize mouse tissue?

I apologize for the delay in my reply. We have not tested this antibody on mouse samples, so it is possible that the antibody may not react with mouse Oxytocin Receptor. The immunogen is 83% homologous with mouse, but testing a positive control from hu...

Read More

We tried ab87312 one time on stressed mouse brain (supposedly stressed mouse has high expression of OXTR in brain). But it turned out negative. We also did a negative control (no Ab) and it looked the same as with the Ab. We don't want to think the Ab ...

Read More

Thank you for your reply. Aside from brain tissue, we would expect this antibody to give positive staining in a subset of tubular cells in kidney, and trophoblastic cells in placenta. It is possible that the antibody may not be suitable for use on para...

Read More

I am interested, but what I really want to do is not IHC but ICC/IF. In other words, I try to do ICC staining on FFPE tissue samples. If this is still ok. I would like to take the discount offer and provide review once we finish the experiment.

Thank you for your reply. The antibody will be most sensitive to the fixation and embedding process. Even if you are formalin-fixing and paraffin-embedding cell samples, we would consider that IHC-P for the purposes of the testing discount even though ...

Read More



Inquiry: Hi, I have a question about the product ab87312. Has it been tested on FFPE samples for ICC staining? In other words, if I have samples that are formalin fixed and paraffin embedded, can I still use ab87312 for immunofluorescence sta...

Read More

Thank you for contacting us. This product has not yet been tested in formalin fixed paraffin embedded sections.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use ou...

Read More

I am interested in your product, ab87312, anti-oxytocin receptor antibody. I need to use it to stain FFPE tissue sections for ICC staining and imaging. Have you tested this product on paraffin embedded tissue samples? If you have, can you provide a pro...

Read More

Thank you very much for your interest in ab87312.

To our knowledge, ab87312 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab87312 now, test it inIHC-P and submit feedback to us in the form o...

Read More

I would like to test ab87312 in mouse using IHC-Fr.


I am very pleased to hear you would like to accept our offer and test ab87312 in mouse]. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in ...

Read More

I would like to test the anti-Oxytocin Receptor antibody, ab87312, for the purpose of Immunofluorescence staining on mouse brains. The antibody has not been tested for mouse tissues. I'm wondering whether I could use the testing discount and get a pers...

Read More

DISCOUNT CODE: ***********
Expiration date: **************

I am very pleased to hear you would like to accept our offer and test ab87312 in mouse samples. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To r...

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册