Anti-Oxytocin Receptor抗体(ab87312)


  • 产品名称
    Anti-Oxytocin Receptor抗体
    参阅全部 Oxytocin Receptor 一抗
  • 描述
    山羊多克隆抗体to Oxytocin Receptor
  • 宿主
  • 经测试应用
    适用于: WB, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Oxytocin Receptor aa 355-367 (C terminal).


    Database link: P30559
    (Peptide available as ab173096)



Our Abpromise guarantee covers the use of ab87312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Oxytocin Receptor peptide (ab173096).

Approx 75kDa band observed in Rat Uterus lysates, but none in Rat Skeletal Muscle lysates as expected (calculated MW of 42.9kDa according to Rat NP_037003.2.

ELISA Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1:4000
ICC/IF Use a concentration of 5 - 10 µg/ml.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr 1/150.



  • ab87312 staining oxytocin receptor in MCF7 cells treated with oxytocin (ab120186), by ICC/IF. Increase of cytoplasmatic oxytocin receptor localization and correspondent decrease in nuclear oxytocin receptor correlates with increased concentration of oxytocin, as described in literature.
    The cells were incubated at 37°C for 15 minutes in media containing different concentrations of ab120186 (oxytocin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab87312 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab87312 at 5 µg/ml staining Oxytocin Receptor in Human prostate stromal cells by Immunocytochemistry/ Immunofluorescense. Antibody was incubated overnight at 4°C and detected with an AlexaFluor-conjugated secondary antibody.
  • Immunohistochemical analysis of paraffin embedded Human prostatic cancer tissue labeling Oxytocin Receptor with ab87312 at 2 ug/ml.

  • ab87312 staining Oxytocin Receptor in rat brain hypothalamus tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 1 hour at 25°C and then incubated with ab87312 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated donkey anti-goat IgG polyclonal used at a 1/1000 dilution.

    Magnocellular hypothalamus was stained.

    See Abreview


This product has been referenced in:
  • Gong L  et al. The antinociception of oxytocin on colonic hypersensitivity in rats was mediated by inhibition of mast cell degranulation via Ca(2+)-NOS pathway. Sci Rep 6:31452 (2016). IF . Read more (PubMed: 27538454) »

See 1 Publication for this product


The sequence of the peptide is C-RRLGETSASKKSN with a calculated MW of 1536.73 Daltons.

I have asked our product managers to add the blocking peptide for ab87312 Anti-Oxytocin Receptor antibody. This peptide can now be found as ab173096 - Oxytocin Receptor peptide, at the following location:

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Thank you for your reply.

We have not had any feedback from customers using this antibody on mouse samples. The image on our datasheet shows staining in rat hypothalamus. You can also view the expected expression of Oxytocin receptor in mouse...

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I apologize for the delay in my reply. We have not tested this antibody on mouse samples, so it is possible that the antibody may not react with mouse Oxytocin Receptor. The immunogen is 83% homologous with mouse, but testing a positive control from hu...

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Thank you for your reply. Aside from brain tissue, we would expect this antibody to give positive staining in a subset of tubular cells in kidney, and trophoblastic cells in placenta. It is possible that the antibody may not be suitable for use on para...

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Thank you for your reply. The antibody will be most sensitive to the fixation and embedding process. Even if you are formalin-fixing and paraffin-embedding cell samples, we would consider that IHC-P for the purposes of the testing discount even though ...

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Thank you for contacting us. This product has not yet been tested in formalin fixed paraffin embedded sections.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use ou...

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Thank you very much for your interest in ab87312.

To our knowledge, ab87312 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab87312 now, test it inIHC-P and submit feedback to us in the form o...

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I am very pleased to hear you would like to accept our offer and test ab87312 in mouse]. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in ...

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I am very pleased to hear you would like to accept our offer and test ab87312 in mouse samples. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To r...

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