概述
- 产品名称Anti-Oxytocin Receptor抗体
参阅全部 Oxytocin Receptor 一抗 - 描述山羊多克隆抗体to Oxytocin Receptor
- 经测试应用适用于: WB, ELISA, ICC/IF, IHC-P, IHC-Frmore details
- 种属反应性与反应: Rat, Human
预测可用于: Rhesus monkey
- 免疫原
Synthetic peptide corresponding to the C terminal amino acids 355-367 (
C-RRLGETSASKKSN
) of Human Oxytocin Receptor (NP_000907.2).
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Immunizing Peptide (Blocking)
- Related Products
应用
Our Abpromise guarantee covers the use of ab87312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1 - 1/3. Can be blocked with Oxytocin Receptor peptide (ab173096). Preliminary experiments in lysates of cell lines A431 and HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? | |
| ELISA | Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1:4000 | |
| ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
| IHC-P | Use a concentration of 2 µg/ml. | |
| IHC-Fr | 1/150. |
靶标
- 功能Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
- 序列相似性Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
- 细胞定位Cell membrane.
- Information by UniProt
-
数据库链接
- Entrez Gene: 5021 Human
- Entrez Gene: 25342 Rat
- Entrez Gene: 702048 Rhesus monkey
- Omim: 167055 Human
- SwissProt: P30559 Human
- SwissProt: P70536 Rat
- SwissProt: P56494 Rhesus monkey
- Unigene: 2820 Human
see all -
别名
- OT R antibody
- OT-R antibody
- OTR 1 antibody
see all
Anti-Oxytocin Receptor antibody 图像
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Immunocytochemistry/ Immunofluorescence-Anti-Oxytocin Receptor antibody(ab87312)ab87312 staining oxytocin receptor in MCF7 cells treated with oxytocin (ab120186), by ICC/IF. Increase of cytoplasmatic oxytocin receptor localization and correspondent decrease in nuclear oxytocin receptor correlates with increased concentration of oxytocin, as described in literature.
The cells were incubated at 37°C for 15 minutes in media containing different concentrations of ab120186 (oxytocin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab87312 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Immunocytochemistry/ Immunofluorescence - Oxytocin Receptor antibody (ab87312)ab87312 at 5 µg/ml staining Oxytocin Receptor in Human prostate stromal cells by Immunocytochemistry/ Immunofluorescense. Antibody was incubated overnight at 4°C and detected with an AlexaFluor-conjugated secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin Receptor antibody (ab87312)Immunohistochemical analysis of paraffin embedded Human prostatic cancer tissue labeling Oxytocin Receptor with ab87312 at 2 ug/ml.
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Immunohistochemistry (Frozen sections) - Anti-Oxytocin Receptor antibody (ab87312)ab87312 staining Oxytocin Receptor in rat brain hypothalamus tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 1 hour at 25°C and then incubated with ab87312 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated donkey anti-goat IgG polyclonal used at a 1/1000 dilution.
Magnocellular hypothalamus was stained.
Anti-Oxytocin Receptor antibody (ab87312)参考文献
ab87312 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"