Anti-Oxytocin-neurophysin 1抗体(ab67457)

概述

  • 产品名称Anti-Oxytocin-neurophysin 1抗体
    参阅全部 Oxytocin-neurophysin 1 一抗
  • 描述
    兔多克隆抗体to Oxytocin-neurophysin 1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Horse, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Oxytocin-neurophysin 1 aa 1-100 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P01178

  • 阳性对照
    • This antibody gave a positive signal in Human adult pituitary gland tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab67457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12, 10 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • MGC126890 antibody
    • MGC126892 antibody
    • Neurophysin 1 antibody
    • Neurophysin I antibody
    • Ocytocin antibody
    • OT antibody
    • OT NPI antibody
    • OXT antibody
    • Oxytocin Neurophysin preproprotein antibody
    • Oxytocin prepro (neurophysin I) antibody
    • Oxytocin prepropeptide antibody
    • Oxytocin-neurophysin 1 antibody
    see all

Anti-Oxytocin-neurophysin 1 antibody 图像

  • Anti-Oxytocin-neurophysin 1 antibody (ab67457) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) )
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
    Additional bands at : 10 kDa (possible cleavage fragment).
    We hypothesize that the 12 and 10 kDa bands represent the pro and mature form of Oxytocin, respectively.
  • ICC/IF image of ab67457 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67457 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Oxytocin-neurophysin 1 antibody (ab67457)参考文献

ab67457 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

You are correct that the mature Oxytoxin (CYIQNCPLG)is a nonapeptide and too short to analyse on a WB.

I can confirm that ab67457 recognises the prepropeptide of oxytoxin, also known as Oxytocin-neurophys...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"