Anti-Oxytocin-neurophysin 1抗体(ab2078)

Abreviews (1)Q&A (3)Specific References (4)
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概述

  • 产品名称Anti-Oxytocin-neurophysin 1抗体
    参阅全部 Oxytocin-neurophysin 1 一抗
  • 描述
    兔多克隆抗体to Oxytocin-neurophysin 1
  • 特异性Less than 1% reactivity to vasopressin.
  • 经测试应用适用于: IHC-FoFr, ICC/IF, IHC-P, RIAmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Guinea pig, Human, Pig
  • 免疫原

    Other Immunogen Type corresponding to Oxytocin-neurophysin 1. Immunogen: oxytocin-thyroglobulin

  • 阳性对照
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2

性能

应用

Our Abpromise guarantee covers the use of ab2078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
ICC/IF 1/200.
IHC-P 1/10000.
RIA 1/100000.

靶标

  • 相关性Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
  • 细胞定位Secreted
  • 数据库链接
    see all
  • 别名
    • MGC126890 antibody
    • MGC126892 antibody
    • Neurophysin 1 antibody
    • Neurophysin I antibody
    • Ocytocin antibody
    • OT antibody
    • OT NPI antibody
    • OXT antibody
    • Oxytocin Neurophysin preproprotein antibody
    • Oxytocin prepro (neurophysin I) antibody
    • Oxytocin prepropeptide antibody
    • Oxytocin-neurophysin 1 antibody
    see all

Anti-Oxytocin-neurophysin 1 antibody 图像

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Oxytocin-neurophysin 1 antibody (ab2078)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Oxytocin-neurophysin 1 antibody (ab2078)Image from Ettrup KS et al, J Chem Neuroanat. 2010 May;39(3):151-65. Epub 2009 Dec 28, Fig 2, doi:10.1016/j.jchemneu.2009.12.004.
    Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
    Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and
  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin-neurophysin 1 antibody (ab2078)
    Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin-neurophysin 1 antibody (ab2078)

    ab2078 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2078 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Oxytocin-neurophysin 1 antibody (ab2078)参考文献

This product has been referenced in:
  • Hurtado MD  et al. Salivary peptide tyrosine-tyrosine 3-36 modulates ingestive behavior without inducing taste aversion. J Neurosci 33:18368-80 (2013). Mouse . Read more (PubMed: 24259562) »
  • Zhao DQ & Ai HB Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin receptor and vasopressin 1b receptor in rat brain. PLoS One 6:e23362 (2011). IHC-FrFl ; Rat . Read more (PubMed: 21858088) »

See all 4 Publications for this product

发表研究结果有使用 ab2078？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Oxytocin antibody

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Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 100C 20 min
Sample Human Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde
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提交于 Jul 01 2014

Dear ladies and gentleman, I am interestet in the anti-OT and anti-AVP antibodies from this paper: PLoS One. 2011;6(8):e23362. Epub 2011 Aug 17. Oxytocin and vasopressin involved in restraint water-immersion stress mediated by oxytocin recep...

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Thank you for your inquiry. Unfortunately, we are unable to track down the particular antibodies the authors used when it is not specifically mentioned in the paper. However, the following antibodies might have been used: ab39363: ...

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I am setting up an RIA to measure oxytocin in rabbit serum and thought your ab 2078 would work. On your website it is listed as having been tested for RIA, do you have a protocol available? Any information regarding this application of ab2078 would be ...

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Thank you for your enquiry. I am sorry but we do not have an online RIA protocol. However, I would like to recommmend the following resources: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=716433&dopt=Abstract ...

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Thank you for your quick answer. I know that sometimes antibodies against vasopressin and oxytocin are using for immunostaining of vasotocin and isotocin. I would like to ask you if it's possible to use your antibodies ab8745 (anti-vasopressin) ...

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Thank you for your enquiry regarding our antibodies ab2078 and ab8745. Unfortunately we do not have any further information regarding their cross reactivity with vasotocin or isotocin, this has not been tested in our laboratories. I'm very sorry ...

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