- 数据表
- 具体的参考文献 (8)
- 实验方案
概述
-
产品名称Anti-Oxytocin-neurophysin 1抗体
参阅全部 Oxytocin-neurophysin 1 一抗 -
描述兔多克隆抗体to Oxytocin-neurophysin 1
-
宿主Rabbit
-
特异性Less than 1% reactivity to vasopressin.
-
经测试应用适用于: IHC-FoFr, ICC/IF, IHC-Pmore details
-
种属反应性与反应: Mouse, Rat, Sheep, Rabbit, Guinea pig, Human, Pig
-
免疫原
Other Immunogen Type corresponding to Oxytocin-neurophysin 1. Immunogen: oxytocin-thyroglobulin
-
阳性对照
- ICC/IF: HepG2 cells.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液Preservative: None
Constituents: Whole Serum -
纯度Whole antiserum
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
-
Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab2078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20043984 | |
| ICC/IF | 1/200. | |
| IHC-P | 1/10000. |
靶标
-
相关性Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
-
细胞定位Secreted
-
数据库链接
- Entrez Gene: 5020 Human
- Entrez Gene: 18429 Mouse
- Entrez Gene: 25504 Rat
- Omim: 167050 Human
- SwissProt: P01178 Human
- SwissProt: P35454 Mouse
- SwissProt: P01177 Pig
- SwissProt: P13389 Sheep
see all -
别名
- MGC126890 antibody
- MGC126892 antibody
- Neurophysin 1 antibody
see all
图片
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Oxytocin-neurophysin 1 antibody (ab2078)Image from Ettrup KS et al, J Chem Neuroanat. 2010 May;39(3):151-65. Epub 2009 Dec 28, Fig 2, doi:10.1016/j.jchemneu.2009.12.004.Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and -
Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin-neurophysin 1 antibody (ab2078)ab2078 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2078 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
文献
This product has been referenced in:
- Calhan OY & Seyrantepe V Mice with Catalytically Inactive Cathepsin A Display Neurobehavioral Alterations. Behav Neurol 2017:4261873 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28133419) »
- Xu H et al. The function of oxytocin: a potential biomarker for prostate cancer diagnosis and promoter of prostate cancer. Oncotarget 8:31215-31226 (2017). Read more (PubMed: 28415720) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"