Anti-OXCT1抗体(ab105320)

概述

  • 产品名称Anti-OXCT1抗体
    参阅全部 OXCT1 一抗
  • 描述
    兔多克隆抗体to OXCT1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 171-434 of Human OXCT1 (BC009001).

  • 阳性对照
    • Human fetal heart and brain lysates; Human fetal kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab105320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
  • 组织特异性Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
  • 通路Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
  • 疾病相关Succinyl-CoA:3-oxoacid CoA transferase deficiency
  • 序列相似性Belongs to the 3-oxoacid CoA-transferase family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 oxoacid CoA transferase 1 antibody
    • 3-oxoacid CoA-transferase 1 antibody
    • EC 2.8.3.5 antibody
    • OTTHUMP00000120012 antibody
    • OTTHUMP00000221550 antibody
    • OXCT antibody
    • Oxct1 antibody
    • SCOT antibody
    • Scot S antibody
    • Scot-S antibody
    • SCOT1_HUMAN antibody
    • Somatic type succinyl CoA:3 oxoacid CoA transferase antibody
    • Somatic-type succinyl-CoA:3-oxoacid CoA-transferase antibody
    • Succinyl CoA:3 ketoacid CoA transferase antibody
    • Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial antibody
    • Succinyl CoA:3 oxoacid CoA transferase antibody
    • Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial antibody
    see all

Anti-OXCT1 antibody 图像

  • All lanes : Anti-OXCT1 antibody (ab105320) at 1/1000 dilution

    Lane 1 : HEK293 whole cell lysate with SEA BLOCK blocking buffer
    Lane 2 : Mitochondria isolated from HEK293 cells with SEA BLOCK blocking buffer
    Lane 3 : Mitochondria isolated from non-synaptosomal human brain tissue. with SEA BLOCK blocking buffer
    Lane 4 : Mitochondria isolated from synaptosomal human neural tissue. with SEA BLOCK blocking buffer
    Lane 5 : Non-synaptosomal murine brain mitochondria (-ve control) with SEA BLOCK blocking buffer
    Lane 6 : Synaptosomal murine neural mitochondria (-ve control) with SEA BLOCK blocking buffer
    Lane 7 : STHdh-Q7/Q7 mitochondria (-ve control) with SEA BLOCK blocking buffer
    Lane 8 : STHdh-Q111/Q111 mitochondria (-ve control) with SEA BLOCK blocking buffer

    Lysates/proteins at 20 µg per lane.
    Blocking peptides at 100 % per lane.

    Secondary
    Goat anti-rabbit IRDye® 800CW at 1/30000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Additional bands at : 20 kDa (possible non-specific binding),25 kDa (possible non-specific binding),52 kDa (possible post-translational modification),70 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    Image is courtesy of an AbReview submitted by Svitlana Yablonska.

    See Abreview

  • All lanes : Anti-OXCT1 antibody (ab105320) at 1/500 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal brain lysate


    Predicted band size : 56 kDa
  • ab105320 at 1/100 dilution staining OXCT1 in Human fetal kidney tissue

Anti-OXCT1 antibody (ab105320)参考文献

ab105320 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - other (Mice brain, and cell line mitochondria)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 20 µg
Specification Mice brain, and cell line mitochondria
Blocking step SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Svitlana Yablonska

Verified customer

提交于 Aug 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"