产品名称 Anti-OXCT1抗体 参阅全部 OXCT1 一抗
WB, IHC-P more details
Recombinant fragment, corresponding to amino acids 171-434 of Human OXCT1 (BC009001).
Human fetal heart and brain lysates; Human fetal kidney tissue.
形式 Lyophilised:Reconstitute in 200ul sterile water
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
1/100 - 1/500.
功能 Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
组织特异性 Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
通路 Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
疾病相关 Succinyl-CoA:3-oxoacid CoA transferase deficiency
序列相似性 Belongs to the 3-oxoacid CoA-transferase family.
细胞定位 Mitochondrion matrix.
Information by UniProt
3 oxoacid CoA transferase 1 antibody
3-oxoacid CoA-transferase 1 antibody
EC 220.127.116.11 antibody
Anti-OXCT1 antibody 图像
Western blot - Anti-OXCT1 antibody (ab105320) Image is courtesy of an AbReview submitted by Svitlana Yablonska.
All lanes : Anti-OXCT1 antibody (ab105320) at 1/1000 dilution Lane 1 : HEK293 whole cell lysate with SEA BLOCK blocking buffer Lane 2 : Mitochondria isolated from HEK293 cells with SEA BLOCK blocking buffer Lane 3 : Mitochondria isolated from non-synaptosomal human brain tissue. with SEA BLOCK blocking buffer Lane 4 : Mitochondria isolated from synaptosomal human neural tissue. with SEA BLOCK blocking buffer Lane 5 : Non-synaptosomal murine brain mitochondria (-ve control) with SEA BLOCK blocking buffer Lane 6 : Synaptosomal murine neural mitochondria (-ve control) with SEA BLOCK blocking buffer Lane 7 : STHdh-Q7/Q7 mitochondria (-ve control) with SEA BLOCK blocking buffer Lane 8 : STHdh-Q111/Q111 mitochondria (-ve control) with SEA BLOCK blocking buffer Lysates/proteins at 20 µg per lane. Blocking peptides at 100 % per lane. Secondary Goat anti-rabbit IRDye® 800CW at 1/30000 dilution Performed under reducing conditions. Predicted band size : 56 kDa Additional bands at : 20 kDa (possible non-specific binding),25 kDa (possible non-specific binding),52 kDa (possible post-translational modification),70 kDa (possible non-specific binding). Exposure time : 5 minutes Image is courtesy of an AbReview submitted by Svitlana Yablonska.
Western blot - OXCT1 antibody (ab105320)
All lanes : Anti-OXCT1 antibody (ab105320) at 1/500 dilution Lane 1 : Human fetal heart lysate Lane 2 : Human fetal brain lysate Predicted band size : 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OXCT1 antibody (ab105320)
ab105320 at 1/100 dilution staining OXCT1 in Human fetal kidney tissue
Anti-OXCT1 antibody (ab105320)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"