Oxaloacetate Assay试剂盒(ab83428)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
概述
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产品名称
Oxaloacetate Assay试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 2 µM -
范围
2 µM - 200 µM -
检测时间
0h 40m -
产品概述
Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (λmax= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
Visit our FAQs page for tips and troubleshooting.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K659 Oxaloacetate Colorimetric/Fluorometric Assay Kit. K659-100 is the same size as the 100 test size of ab83428.
Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Developer 1 vial OAA Assay Buffer 1 x 25ml OAA Enzyme Mix 1 vial OAA Probe (in DMSO) 1 x 200µl OAA Standard (10 µmol) 1 vial -
研究领域
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相关性
Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. -
别名
- OAA
- Oxaloacetic acid
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab83428 被引用在 9 文献中.
- Fiorito V et al. The heme synthesis-export system regulates the tricarboxylic acid cycle flux and oxidative phosphorylation. Cell Rep 35:109252 (2021). PubMed: 34133926
- Yan S et al. Discovery of GOT1 Inhibitors from a Marine-Derived Aspergillus terreus That Act against Pancreatic Ductal Adenocarcinoma. Mar Drugs 19:N/A (2021). PubMed: 34822459
- Ding H et al. NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA. Cancer Cell Int 20:425 (2020). PubMed: 32884448
- Wang J et al. miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1. Biochem Biophys Res Commun 509:241-248 (2019). PubMed: 30591220
- Jiang H et al. The Mitochondria-Targeted Metabolic Tubular Injury in Diabetic Kidney Disease. Cell Physiol Biochem 52:156-171 (2019). PubMed: 30816665
- Sun W et al. Aspulvinone O, a natural inhibitor of GOT1 suppresses pancreatic ductal adenocarcinoma cells growth by interfering glutamine metabolism. Cell Commun Signal 17:111 (2019). PubMed: 31470862
- Park S et al. High activity and stability of codon-optimized phosphoenolpyruvate carboxylase from Photobacterium profundum SS9 at low temperatures and its application for in vitro production of oxaloacetate. Bioprocess Biosyst Eng 37:331-5 (2014). PubMed: 23719931
- Park S et al. Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina. Bioprocess Biosyst Eng 36:127-31 (2013). PubMed: 22644065
- Long LH & Halliwell B The effects of oxaloacetate on hydrogen peroxide generation from ascorbate and epigallocatechin gallate in cell culture media: potential for altering cell metabolism. Biochem Biophys Res Commun 417:446-50 (2012). Functional Studies . PubMed: 22166196