概述

  • 产品名称
    Oxaloacetate Assay试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Quantitative
  • 灵敏度
    > 2 µM
  • 范围
    2 µM - 200 µM
  • 检测时间
    0h 40m
  • 产品概述

    Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (?max= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase

  • 经测试应用
    适用于: Functional Studiesmore details

性能

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应用

Our Abpromise guarantee covers the use of ab83428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Oxaloacetate fluorimetrically measured in biofluids (after spin column) showing concentration (micromolar)

  • Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted

  • Oxaloacetate standard curve generated following the kit protocol.

实验方案

文献

This product has been referenced in:
  • Park S  et al. Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina. Bioprocess Biosyst Eng 36:127-31 (2013). Read more (PubMed: 22644065) »
  • Long LH & Halliwell B The effects of oxaloacetate on hydrogen peroxide generation from ascorbate and epigallocatechin gallate in cell culture media: potential for altering cell metabolism. Biochem Biophys Res Commun 417:446-50 (2012). Functional Studies . Read more (PubMed: 22166196) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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