Anti-OVOL2抗体(ab83265)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to OVOL2
  • 经测试应用
    适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 226-275 (QEDLYLHVNS AHPGSSFLKK TSKKLAALLQ GKLTSAHQEN TSLSEEEERK) of human OVOL2 (NP_067043).

  • 阳性对照
    • 721_B cell lysate; human cornea

性能

应用

Our Abpromise guarantee covers the use of ab83265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:62500.

IHC-Fr 1/250.

靶标

  • 功能
    DNA-binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. Probably acts as a transcription regulator.
  • 组织特异性
    Expressed in testis, ovary, heart and skeletal muscle.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA504H3.3 antibody
    • EUROIMAGE566589 antibody
    • hOvo 2 antibody
    • hOvo2 antibody
    • Ovo like 2 (Drosophila) antibody
    • Ovo like 2 antibody
    • ovo-like zinc finger 2 antibody
    • OVOL 2 antibody
    • OVOL2 antibody
    • OVOL2_HUMAN antibody
    • Transcription factor Ovo like 2 antibody
    • Transcription factor Ovo-like 2 antibody
    • Zinc finger protein 339 antibody
    • ZNF 339 antibody
    • ZNF339 antibody
    see all

Anti-OVOL2 antibody 图像

  • Anti-OVOL2 antibody (ab83265) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Gel concentration: 12%
  • Immunofluorescent staining of OVOL2 in human corneal endothelium with ab83265 at a dilution of 1/250. a: signal for Propidium iodide and OVOL2 b: signal for OVOL2 only c: corneal epithelium.

    Corneal epithelium as well as limbal epithelium was positive in the cytoplasm. Stromal keratocytes and endothelium was positive predominantly in the cell nucleus

Anti-OVOL2 antibody (ab83265)参考文献

ab83265 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (P21 cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
Yes - 0.2% Tween 20
Specification
P21 cornea
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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提交于 Oct 04 2017

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