概述

  • 产品名称
  • 描述
    兔多克隆抗体to OVGP1
  • 经测试应用
    适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
    预测可用于: Rat
  • 免疫原

    An 18 amino acid peptide from near the amino terminus of Human OVGP1.

  • 阳性对照
    • WB: 3T3 cell lysate IHC-P: Human fallopian tube

性能

应用

Our Abpromise guarantee covers the use of ab118590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.Can be blocked with OVGP1 peptide (ab188397).
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.
  • 组织特异性
    Oviduct.
  • 序列相似性
    Belongs to the glycosyl hydrolase 18 family.
  • 细胞定位
    Cytoplasmic vesicle > secretory vesicle. Secretory granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHIT5 antibody
    • EGP antibody
    • Estrogen dependent oviduct protein antibody
    • Estrogen-dependent oviduct protein antibody
    • MUC9 antibody
    • mucin 9 antibody
    • Mucin-9 antibody
    • OGP antibody
    • OVGP 9 antibody
    • Ovgp1 antibody
    • OVGP1_HUMAN antibody
    • Oviduct glycoprotein antibody
    • Oviduct-specific glycoprotein antibody
    • Oviductal glycoprotein antibody
    • Oviductin antibody
    see all

Anti-OVGP1 antibody 图像

  • ab118590 staining OVGP1 in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA + PBST (PBS + 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab118590, at 5 µg/ml, staining OVGP1 in formalin fixed, paraffin embedded Human fallopian tube by Immunohistochemistry.

Anti-OVGP1 antibody (ab118590)参考文献

This product has been referenced in:
  • Chen S  et al. Long-term culture of primary porcine oviduct epithelial cells: validation of a comprehensive in vitro model for reproductive science. Theriogenology 80:862-9 (2013). Read more (PubMed: 23973051) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Cow Cell (Bovine oviductal epithelial cell culture (BOEC))
Specification
Bovine oviductal epithelial cell culture (BOEC)
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Jan 16 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Cow Tissue sections (Oviduct)
Specification
Oviduct
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Dec 17 2013

Thank you for confirming this information. I am pleased to help answer your questions:

1. How much volume is in the vial?
From the datasheet: We sell 50 ug per vial at 1 mg ml.
Can work out the volume using regular laboratory maths...

Read More

Thank you for your inquiry.

I can confirm that one vial of contains 50ug with a concentration of 1mg/ml.

As a starting working solution for WB we recommend a concentration of 1ug/ml that is a 1:1000 dilution.

For IHC-P we ...

Read More

Thank you for your enquiry.

I can confirm that each vial of this antibody contains 50 ug of antibody. The concentration of the antibdoy is 1 mg/ml (1000ug/ml or 1ug/ul).

This is stated on the datasheet as 50 ug at 1 mg/ml
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册