概述

  • 产品名称Anti-OVGP1抗体
    参阅全部 OVGP1 一抗
  • 描述
    兔多克隆抗体to OVGP1
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
    预测可用于: Rat
  • 免疫原

    An 18 amino acid peptide from near the amino terminus of Human OVGP1.

  • 阳性对照
    • WB: 3T3 cell lysate IHC-P: Human fallopian tube

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab118590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.Can be blocked with OVGP1 peptide (ab188397).
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.
  • 组织特异性Oviduct.
  • 序列相似性Belongs to the glycosyl hydrolase 18 family.
  • 细胞定位Cytoplasmic vesicle > secretory vesicle. Secretory granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHIT5 antibody
    • EGP antibody
    • Estrogen dependent oviduct protein antibody
    • Estrogen-dependent oviduct protein antibody
    • MUC9 antibody
    • mucin 9 antibody
    • Mucin-9 antibody
    • OGP antibody
    • OVGP 9 antibody
    • Ovgp1 antibody
    • OVGP1_HUMAN antibody
    • Oviduct glycoprotein antibody
    • Oviduct-specific glycoprotein antibody
    • Oviductal glycoprotein antibody
    • Oviductin antibody
    see all

Anti-OVGP1 antibody 图像

  • ab118590 staining OVGP1 in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA + PBST (PBS + 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab118590, at 5 µg/ml, staining OVGP1 in formalin fixed, paraffin embedded Human fallopian tube by Immunohistochemistry.

Anti-OVGP1 antibody (ab118590)参考文献

This product has been referenced in:
  • Chen S  et al. Long-term culture of primary porcine oviduct epithelial cells: validation of a comprehensive in vitro model for reproductive science. Theriogenology 80:862-9 (2013). Read more (PubMed: 23973051) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Cow Cell (Bovine oviductal epithelial cell culture (BOEC))
Specification Bovine oviductal epithelial cell culture (BOEC)
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Jan 16 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Cow Tissue sections (Oviduct)
Specification Oviduct
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Dec 17 2013

Thank you for confirming this information. I am pleased to help answer your questions:

1. How much volume is in the vial?
From the datasheet: We sell 50 ug per vial at 1 mg ml.
Can work out the volume using regular laboratory maths...

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Thank you for your inquiry.

I can confirm that one vial of contains 50ug with a concentration of 1mg/ml.

As a starting working solution for WB we recommend a concentration of 1ug/ml that is a 1:1000 dilution.

For IHC-P we ...

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Thank you for your enquiry.

I can confirm that each vial of this antibody contains 50 ug of antibody. The concentration of the antibdoy is 1 mg/ml (1000ug/ml or 1ug/ul).

This is stated on the datasheet as 50 ug at 1 mg/ml
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"