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Our Abpromise guarantee covers the use of ab118590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.Can be blocked with OVGP1 peptide (ab188397).|
|IHC-P||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
ab118590 staining OVGP1 in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA + PBST (PBS + 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.